Primer

Part:BBa_K268018:Design

Designed by: Nora Yucel   Group: iGEM09_UChicago   (2009-10-21)

YOR049C -> GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2459
    Illegal PstI site found at 1223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2459
    Illegal PstI site found at 1223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2459
    Illegal BglII site found at 1013
    Illegal BamHI site found at 42
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2459
    Illegal PstI site found at 1223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2459
    Illegal PstI site found at 1223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 702


Design Notes

Source

Promoter region from gene YOR049C in S. cerevisiae as obtained through yeastgenome.org. Gene deletion and tagging cassette from Longtine, et al (1998).

References